Probing the Catalytically Active Species in POM‐Catalysed DNA‐Model Hydrolysis**

Probing substrate interactions in the active tunnel of a catalytically deficient cellobiohydrolase (Cel7).

Cellobiohydrolases break down cellulose sequentially by sliding along the crystal surface with a single cellulose strand threaded through the catalytic tunnel of the enzyme. This so-called processive mechanism relies on a complex pattern of enzyme-substrate interactions, which need to be addressed in molecular descriptions of processivity and its driving forces. Here, we have used titration cal...

PROBING THE ACTIVE SITE OF RHODANESE WITH DISULFIDE REAGENTS

Isolation and Properties of the Catalytically Active

Nonactivated phosphorylase kinase was dissociated to catalytically active lower molecular weight species by incubation with either ATP or LiBr at 0°C. Sucrose density gradient analysis showed that incubation with 100 m~ ATP produced 14 S and 7.5 S active forms and incubation with 1 M LiBr produced a 5 S active species. Large increases in the pH 6.8/8.2 activity ratio and loss of inhibition by E...

Modeling of catalytically active metal complex species and intermediates in reactions of organic halides electroreduction.

The results of quantum chemical modeling of organic and metal-containing intermediates that occur in electrocatalytic dehalogenation reactions of organic chlorides are presented. Modeling of processes that take place in successive steps of the electrochemical reduction of representative C1 and C2 chlorides - CHCl3 and Freon R113 (1,1,2-trifluoro-1,2,2-trichloroethane) - was carried out by densi...

Active Probing

Problem determination is one of the most important tasks in managing distributed systems. Probing (both at the transaction and network levels) has been widely used for assessing compliance with Service Level Agreements and locating problems in distributed systems. However a probing scheme which uses a fixed set of regularly scheduled probes can be expensive in terms of the number of synthetic t...